Data-enriched characterization of cell populations. Morphological facts from photos can include towards the richness of flow cytometry information. As an example, the determine demonstrates an in any other case common apoptosis assay working with Annexin V and PI, introducing cell imaging to characterize cells in Each individual inhabitants to reveal morphologically distinctive capabilities. These insights couldn't are already attained from multiplex staining by itself.
As samples are acquired about the Attune CytPix Flow Cytometer, the large-speed brightfield camera captures and retailers illustrations or photos of detected occasions in a level around six,000 photos for each next, based on the flow fee and image size. For higher overall flexibility, Attune Cytometric Software enables you to modify the impression seize frequency as desired and select a specific gate for image seize, if expected.
Incorporate acoustic focusing engineering with large-parameter Investigation for flexible spectral unmixing and conventional workflows.
Explore new insights into your experimental data by visualizing populations applying put together fluorescence and morphology information. Our software interface wizards help downsampling, concatenation (merged information) and dimensionality applications which can be built into your program for an intuitive, streamlined details Investigation knowledge.
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Listed here, a seasoned consumer has gated singlets confidently. Soon after evaluating the manual singlet gate, the CytPix picture-derived parameter ParticleCount reveals this gate is made up of more than four% aggregates.
Pair your Attune flow cytometer by having an autosampler for greater workflow performance, and insert robotic integration for max throughput.
Constant image top quality even at higher throughput Acoustic focusing and a substantial-speed digicam Blend to graphic these T cells regularly at website minimal or substantial flow costs. Easily change emphasis and digital camera settings to satisfy experimental demands.
Early indications of this contamination experienced originally been dismissed as particles. Backgating to discover new populations. Backgating imaged cells about the Attune CytPix Flow Cytometer also means that you can use morphological capabilities to discover interesting subpopulations that may not be obvious from flow cytometry details on your own.
Buyers can watch function images in real-time to verify sample top quality, enhance protocols, and record the morphology of every mobile populace recognized for Assessment. Imaging seize also Advantages from acoustic concentrating, which really helps to place the cells so that a pointy, centered image is received.
Sound waves allow exact mobile alignment, leading to Excellent facts high-quality and ~10X more rapidly sample flow costs.
Software note: Automatic impression Examination lessens consumer-to-person variability in flow cytometry gating methods
Leverage the power of Highly developed engineering and innovation to speed up your discoveries. Rely on while in the Attune Flow Cytometer relatives to provide the trustworthiness, velocity, and precision that established your work aside.
No wash/no lyse leukocyte analysis with imaging. Cells ended up obtained from 24-hour-aged blood by dilution in one mM EDTA ( Specialist UX/UI enables end users to educate AI models for expanded purposes. (A) The “Teach Design” characteristic offers annotation instruments and true-time model schooling accuracy.
Gating on these extended parameters permits you to swiftly and properly identify populations of curiosity and confirm gating technique with little or no manual review. The Attune software delivers usage of state-of-the-art equipment Discovering Examination resources within just an expertly intended consumer interface, which makes it exceptionally simple to operate.
Since the mobile line experienced previously been analyzed about the Attune CytPix Flow Cytometer, the scientists went again to the pictures and ended up capable to document the microbial an infection at the very least five days previously.
The Attune CytPix Flow Cytometer lets users to visualize samples through acquisition, verify the accuracy of gating approaches, and aid results with trustworthy documentation for publication purposes.
What gave the impression to be a homogeneous population is definitely a lot more numerous—an insight that should be regarded when interpreting success.